Nextclade: analysis of viral genetic sequences

Nextclade is an open-source project for viral genome alignment, mutation calling, clade assignment, quality checks and phylogenetic placement.

Nextclade consists of a set of related tools:

  • Nextclade Web - a web application available online at clades.nextstrain.org

  • Nextclade CLI - a command-line tool that mimics Nextclade Web

  • Nextalign CLI - a command-line tool which is similar to Nextclade CLI, but performs only the alignment, without further analysis

All tools are powered by the same algorithms, they consume the same inputs and produce the same outputs, but they differ in the user interface, the amount of features included and the degree of customization. It is recommended to start with Nextclade Web and later proceed to CLI tools if you have more advanced use-cases (for example, repeated batch processing, bioinformatics pipelines).

Nextclade is a part of Nextstrain, an open-source project to harness the scientific and public health potential of pathogen genome data. All source code is available on GitHub.

Note

Do you find Nextclade useful? Tell us about your use-case and experience with it.

For a general conversation, feel free to join Nextstrain Discussion at discussion.nextstrain.org.

If you want to report an error or request a new feature, please open a new Github Issue (despite the name, “Github Issues” can be about anything, not only problems).

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